The biological functions of the human body are complex and over the years, scientists and researchers have carried out research to discover the human anatomy. The major building block of the human body is the cell. They are the structural compositions of the body and are mostly referred to as the smallest basic units of life. In order to obtain a more comprehensive understanding of the functioning of these units, live cell microscopy is applied.
This imaging method has been accessible for many years now and has been extended to many different fields. It involves viewing of these structural units under a time-lapse microscope in order to study their cellular structure. Since its pioneering, several methods of such imaging have been developed which allows these units to be studied in great detail. This enhances the knowledge on functions of cellular structures and tissues.
In order for great results to be obtained, the cell under observation should be well maintained. The temperature of the environment should be kept stable. Stability is maintained at 37 degrees for mammalian cellular structures. It is important to use a large microscope as its enclosures offer great temperature stability. The osmolarity of the environment should be avoided. It is caused by evaporation of medium and it is therefore important that the air around the sample is humidified. The mammalian cells require a bicarbonate-based culture media to maintain physiological pH. This will allow capturing of good quality images.
In order to obtain live-cell fluorescent imaging, biologists need to obtain as much fluorescent light in a bid to reduce the incident light that may cause phototoxic effects. Since many cells and tissues are exposed to light in their life cycle, it is important for imaging applications to reduce exposure to light. To ensure this, microscopes should be able to collect as much light as possible.
Lowering background light during such experiments can be done in various ways. This imaging system is able to reduce illumination time and the amount by enhancing the effectiveness of the optical path in use. Shuttling off the illuminating light when not in use is also reduces damage induced by light. The fluorescent light used in illuminating the specimen should not be contaminated by ultraviolet or infrared lights.
This light reduction can be achieved by measures like improving path for optical light to the device. Additionally, the detector optimization of signal and noises should be maximized so as to illuminate the available light in the most satisfying manner. Another way is using a device that has a sensitive detector in order to sense and eliminate incident light.
When choosing an optimal imaging system, there are several factors to be considered. These factors include the sensitivity of the detector, the viability of specimen and the required speed for image acquisition. It should make maximum use of light and also use few optical elements in the path of light. Many different types of fluorescent proteins of different colors are available to be used as fluorescent tags.
The benefits from such experiments include examples like monitoring molecular interaction, viral replication, and testing temperature dependency of drug-induced events. It is however disadvantageous since the cellular structures are damaged at the end of an experiment.
This imaging method has been accessible for many years now and has been extended to many different fields. It involves viewing of these structural units under a time-lapse microscope in order to study their cellular structure. Since its pioneering, several methods of such imaging have been developed which allows these units to be studied in great detail. This enhances the knowledge on functions of cellular structures and tissues.
In order for great results to be obtained, the cell under observation should be well maintained. The temperature of the environment should be kept stable. Stability is maintained at 37 degrees for mammalian cellular structures. It is important to use a large microscope as its enclosures offer great temperature stability. The osmolarity of the environment should be avoided. It is caused by evaporation of medium and it is therefore important that the air around the sample is humidified. The mammalian cells require a bicarbonate-based culture media to maintain physiological pH. This will allow capturing of good quality images.
In order to obtain live-cell fluorescent imaging, biologists need to obtain as much fluorescent light in a bid to reduce the incident light that may cause phototoxic effects. Since many cells and tissues are exposed to light in their life cycle, it is important for imaging applications to reduce exposure to light. To ensure this, microscopes should be able to collect as much light as possible.
Lowering background light during such experiments can be done in various ways. This imaging system is able to reduce illumination time and the amount by enhancing the effectiveness of the optical path in use. Shuttling off the illuminating light when not in use is also reduces damage induced by light. The fluorescent light used in illuminating the specimen should not be contaminated by ultraviolet or infrared lights.
This light reduction can be achieved by measures like improving path for optical light to the device. Additionally, the detector optimization of signal and noises should be maximized so as to illuminate the available light in the most satisfying manner. Another way is using a device that has a sensitive detector in order to sense and eliminate incident light.
When choosing an optimal imaging system, there are several factors to be considered. These factors include the sensitivity of the detector, the viability of specimen and the required speed for image acquisition. It should make maximum use of light and also use few optical elements in the path of light. Many different types of fluorescent proteins of different colors are available to be used as fluorescent tags.
The benefits from such experiments include examples like monitoring molecular interaction, viral replication, and testing temperature dependency of drug-induced events. It is however disadvantageous since the cellular structures are damaged at the end of an experiment.
About the Author:
When you are searching for information about live cell microscopy, come to our web pages online today. More details are available at http://www.invivoscientific.com/about-us now.
No comments:
Post a Comment